New lab pub: The Phosphotransfer Protein CD1492 Represses Sporulation Initiation in Clostridium difficile

In Infect Immun, news, PubMed, Uncategorized by smcbride

The Phosphotransfer Protein CD1492 Represses Sporulation Initiation in Clostridium difficile
Childress KO, Edwards AN, Nawrocki KL, Woods EC, Anderson SE, McBride SM

The formation of spores is critical for survival of Clostridium difficile outside of the host gastrointestinal tract. Persistence of C. difficile spores greatly contributes to the spread of C. difficile infection (CDI), and the resistance of spores to antimicrobials facilitates the relapse of infection. Despite the importance of sporulation to C. difficile pathogenesis, the molecular mechanisms controlling spore formation are not well understood. The initiation of sporulation is known to be regulated through activation of the conserved transcription factor, Spo0A. Multiple regulators influence Spo0A activation in other species; however, many of these factors are not conserved in C. difficile, and few novel factors have been identified. Here, we investigate the function of a protein, CD1492, which is annotated as a kinase and originally proposed to promote sporulation by directly phosphorylating Spo0A. We found that deletion of CD1492 results in increased sporulation, indicating that CD1492 is a negative regulator of sporulation. Accordingly, we observed increased transcription of Spo0A-dependent genes in the CD1492 mutant. Deletion of CD1492 also resulted decreased toxin production in vitro and in decreased virulence in the hamster model of CDI. Further, the CD1492 mutant demonstrated effects on gene expression that are not associated with Spo0A activation, including lower sigD and rstA transcription, suggesting that this protein interacts with factors other than Spo0A. Altogether, the data indicate that CD1492 negatively affects sporulation and positively influences motility and virulence. These results provide further evidence that C. difficile sporulation is regulated differently from that of other endospore forming species.