June 2012 – Present
My undergraduate and doctoral research interests and training have primarily revolved around RNA regulation. My previous training has provided me with a strong foundation in genetics and regulation, utilizing genetic, biochemical and molecular biological techniques. My undergraduate research in Dr. Sidney Kushner’s lab at the University of Georgia involved the characterization of the role of two exoribonucleases in RNA degradation in Escherichia coli.
My dissertation studies were conducted in Dr. Tony Romeo’s lab at Emory University. To expand our knowledge of the global regulatory role of the RNA binding protein, CsrA, in E. coli, I designed a biochemical method to isolate RNA that copurified with recombinant histidine-tagged CsrA to use along with cDNA synthesis and deep sequencing to determine which RNAs directly interact with CsrA in vivo. From here, we embarked on an extensive study detailing transcriptional and posttranscriptional regulatory interactions between two complex regulatory systems, the Csr and stringent response systems.
From 2010 until 2012, I trained as a postdoctoral fellow in Justin Gallivan’s lab in the Department of Chemistry at Emory University. Here, my research focused on the function of synthetic theophylline riboswitches in E. coli in various phases of growth and on the in vitro and in vivo selection of a novel aptamer and riboswitch that recognizes the neurotoxin, paraoxon.
In the McBride lab, my research will focus on elucidating the genetic pathways that regulate Clostridium difficile colonization.